Publications

Alphey, M.S., Wolford, C.B. and MacNeill, S.A. (2023) Canonical binding of Chaetomium thermophilum DNA polymerase δ/ζ subunit PolD3 and flap endonuclease Fen1 to PCNA. Front. Mol. Biosci. doi: 10.3389/fmolb.2023.1320648. PubMed

Hacker, C., Sendra, K., Keisham, P., Filipescu, T., Lucocq, J., Salimi, F., Ferguson, S., Bhella, D., MacNeill, S.A., Embley, M. and Lucocq, J. (2023) Biogenesis, inheritance and 3D ultrastructure of the microsporidian mitosome. Life Science Alliance 7, e202201635. PubMed

Yang, D., Alphey, M.S. and MacNeill, S.A. (2023) Non-canonical binding of the Chaetomium thermophilum PolD4 N-terminal PIP motif to PCNA involves Q-pocket and compact 2-fork plug interactions but no 310 helix. FEBS J. 290, 162-175. PubMed

MacNeill, S.A. (2021) Remote homology detection identifies a eukaryotic RPA DBD-C-like DNA binding domain as a conserved feature of archaeal Rpa1-like proteins. Front. Mol. Biosci. doi: 10.3389/fmolb.2021.675229. PubMed

Taib, N., Gribaldo, S. and MacNeill, S.A. (2021) Single-stranded DNA binding proteins in the Archaea. Methods Mol. Biol. 2281: 23-47. PubMed

Longmuir, S., Akhtar, N. and MacNeill, S.A. (2019) Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR-Cas9 mediated gene deletion. BMC Res. Notes 12, 191. PubMed

Tonini, M.L., Peña-Diaz, P., Haindrich, A.C., Basu, S., Pierik, A.J., Lill, R., MacNeill, S.A., Smith, T.K and Lukeš, J. (2018) Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei. PLoS Pathogens 14, e1007326. PubMed

MacNeill, S.A. (2018) The archaeal RecJ-like proteins: nucleases and ex-nucleases with diverse roles in DNA replication and repair. Emerg. Top. Life Sci. 2, 493-501. PubMed

Kipandula, W., Young, S.A., MacNeill, S.A. and Smith, T.K. (2018) Screening of the MMV and GSK open access chemical boxes using a viability assay developed against the kinetoplastid Crithidia fasciculata. Mol. Biochem. Parasitol. 222, 61-69. PubMed

Knüppel, R., Christensen, R.H., Gray, F.C., Esser, D., Strauß, D., Medenbach, J., Siebers, B., MacNeill, S.A., LaRonde, N. and Ferreira-Cerca, S. (2017) Insights into the evolutionary conserved regulation of Rio ATPase activity. Nucl. Acids. Res. 46, 1441-1456. PubMed

Kipandula, W., Smith, T.K. and MacNeill, S.A. (2017) Tandem affinity purification of exosome and replication factor C complexes from the non-human infectious kinetoplastid parasite Crithidia fasciculata. Mol. Biochem. Parasitol. 217, 19-22. PubMed

Varnaite, R. and MacNeill, S.A. (2016) Meet the neighbours: mapping local protein interactomes by proximity-dependent labelling with BioID. PROTEOMICS 16, 2503-2518. PubMed

MacNeill, S.A. (2016) PCNA-binding proteins in the archaea: novel functionality beyond the conserved core. Current Genetics 62, 527-532. PubMed

Giroux, X. and MacNeill, S.A. (2015) A novel archaeal DNA repair factor that acts with the UvrABC system to repair mitomycin C-induced DNA damage in a PCNA-dependent manner. Mol. Micro. 99, 1-14. PubMed

Giroux, X. and MacNeill, S.A. (2015) Inhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5′-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii. FEMS Micro. Letts. 262, pii: fnv181. PubMed

Giroux, X. and MacNeill, S.A. (2015) Molecular genetic methods to study DNA replication protein function in Haloferax volcanii, a model archaeal organism. Methods Mol. Biol. 1300, 187-213. PubMed

MacNeill, S.A. (2014) Identification of a candidate Rad1 subunit for the kinetoplastid 9-1-1 (Rad9-Hus1-Rad1) complex. Biology 3, 922-927. PubMed

Kristensen, T.P., Maria Cherian, R., Gray, F.C. and MacNeill, S.A. (2014) The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the β7-β8 and β9-β10 hairpin loops and conserved zinc binding domain cysteines. Front. Microbiol. 5, 123. PubMed

Onesti, S. and MacNeill, S.A. (2013) Structure and evolutionary origins of the CMG complex. Chromosoma 122, 47-53. PubMed

MacNeill, S.A. (2012) Composition and dynamics of the eukaryotic replisome: a brief overview. Subcell. Biochem. 62, 1-17. PubMed

MacNeill, S.A. (2012) The eukaryotic replisome: a guide to protein structure and function. SpringerLink

Pai, C.C., Schnick, J., MacNeill, S.A. and Kearsey S.E. (2012) Conditional inactivation of replication proteins in fission yeast using hormone-binding domains. Methods 57, 227-233. PubMed

Salguero, I., Guarino, E., Shepherd, M., Deegan, T., Havens, C.G., MacNeill, S.A., Walter, J.C. and Kearsey, S.E. (2012) Ribonucleotide reductase activity is regulated by proliferating cell nuclear antigen (PCNA). Curr. Biol. 22, 720-726. PubMed

Li, J., Schnick, J., Hayles, J and MacNeill, S.A. (2011) Purification and functional inactivation of the fission yeast MCM-BP complex. FEBS Letts. 585, 3850-3855. PubMed

Skowyra, A. and MacNeill, S.A. (2011) Identification of essential and non-essential single-stranded DNA binding proteins in a model archaeal organism. Nucl. Acids Res. 40, 1077-1090. PubMed

MacNeill, S.A. (2011) Protein-protein interactions in the archaeal core replisome. Biochem. Soc. Trans. 39, 163-168. PubMed

Swiatek, A. and MacNeill, S.A. (2010) The archaeo-eukaryotic GINS proteins and the archaeal primase catalytic subunit PriS share a common domain. Biol. Direct 5, 17. PubMed

Johansson, E. and MacNeill, S.A. (2010) The eukaryotic replicative DNA polymerases take shape. Trends Biochem. Sci. 35, 339-347. PubMed

MacNeill, S.A. (2010) Structure and function of the GINS complex, a key component of the eukaryotic replisome. Biochem. J. 425, 489-500. PubMed

Morgunova, K., Gray, F.C., MacNeill, S.A. and Ladenstein, R. (2009) Structural insights into the adaptation of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii to a high salt environment. Acta. Cryst. D 65, 1081-1088. PubMed

Gray, F.C., Whitehead, K.A. and MacNeill, S.A. (2009) Inactivating mutations in the fission yeast replication factor C subunit Rfc2 cluster near the ATP binding site and arginine finger motif. FEBS J. 276, 4803-4813. PubMed

Sanchez Garcia, J., Baranovskiy, A.G., Knatko, E.V., Gray, F.C., Tahirov, T.H. and MacNeill, S.A. (2009) Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis. BMC Mol Biol. 10, 82. PubMed

Akman, G. and MacNeill, S.A. (2009) MCM-GINS and MCM-MCM interactions in vivovisualised by bimolecular fluorescence complementation in fission yeast. BMC Cell Biol. 10, 12. PubMed

Pai, C.-C., Garcia, I., Wang, S.-W., Cotterill, S., MacNeill, S.A. and Kearsey, S.E. (2009) GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast. Mol. Biol. Cell. 20, 1213-22. PubMed

MacNeill, S.A. (2009) The haloarchaeal chromosome replication machinery. Biochem. Soc. Trans. 37, 108-113. PubMed

Boe, C.A., Garcia, I., Pai, C.-C., Sharom, J.R., Skjolberg, H.C., Boye, E., Kearsey, S., MacNeill, S.A., Tyers, M.D. and Grallert, B. (2008) Rapid regulation of protein activity in fission yeast. BMC Cell Biol. 9, 23. PubMed

Zhao, A., Gray, F.C. and MacNeill, S.A. (2006) ATP- and NAD+-dependent DNA ligases share an essential function in the halophilic archaeon Haloferax volcanii. Mol. Microbiol. 59, 743-752. PubMed

Poidevin, L. and MacNeill, S.A. (2006) Biochemical characterisation of LigN, an NAD-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal activity at high salt concentrations. BMC Molecular Biology 7, 44. PubMed

Kim, J., Robertson, K., Mylonas, K.J.L., Gray, F.C., Charapitsa, I. and MacNeill, S.A. (2005) Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully-functional RFC in fission yeast. Nucl. Acids Res. 33, 4078-4089. PubMed

MacNeill, S.A. (2005) Genome stability: A self-sufficient DNA repair machine. Current Biology 15, R21-R23. PubMed

Martin, I.V. and MacNeill, S.A. (2004) Functional analysis of subcellular localisation and protein-protein interaction sequences in the essential DNA ligase I protein of fission yeast. Nucl. Acid Res. 32, 632-642. PubMed

Sanchez Garcia, J., Ciufo, L.F., Yang, X., Kearsey, S. and MacNeill, S.A. (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit. Nucl. Acid Res. 32, 3005-3016. PubMed

Ryu, G.-H., Tanaka, H., Kim, D.-H., Kim, J.-H., Bae, S.-H., Kwon, Y.-N., Rhee, J.S., MacNeill, S.A. and Seo, Y.-S. (2004) Genetic and biochemical analysis of Pfh1 DNA helicase function in fission yeast. Nucl. Acid Res. 32, 4205-4216. PubMed

Tanaka, H., Ryu, G.-H., Seo, Y.-S., and MacNeill, S.A. (2004) Genetic analysis of lagging strand DNA synthesis in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta. Nucl. Acid Res. 32, 6367-6377. PubMed

MacNeill, S.A. (2004) Skimming the surface of the stuff of life. Heredity 93, 318-319.

Gray, F.C., Pohler, J.R.G., Warbrick, E., and MacNeill, S.A. (2004) Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27. BMC Molecular Biology 5, 21. PubMed

Kim, J. and MacNeill, S.A. (2003) Genome stability: A new member of the RFC family. Current Biology 13, R873-R875. PubMed

MacNeill, S.A. (2002) Genome sequencing: And then there were six. Current Biology 12, R294-R296. PubMed

Bermudez, V.P., MacNeill, S.A., Tappin, I. and Hurwitz, J. (2002) The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. J. Biol. Chem. 277, 36853-36862. PubMed

Tanaka, H., Ryu, G.-H., Seo, Y.-S., Tanaka, K., Okayama, H., MacNeill, S.A. and Yuasa, Y. (2002) The fission yeast pfh1 gene encodes an essential 5′ to 3′ DNA helicase required for the completion of S-phase. Nucl. Acids Res. 30, 4728-4739. PubMed

Martin, I.V. and MacNeill, S.A. (2002) ATP-dependent DNA ligases. Genome Biology 3, reviews 3005.1-3005.7. PubMed

MacNeill, S.A. (2001) Understanding the enzymology of archaeal DNA replication: progress in form and function. Molec. Microbiol. 40, 520-529. PubMed

MacNeill, S.A. (2001) DNA replication: Partners in the Okazaki two-step. Current Biology 11, R842-R844. PubMed

MacNeill, S.A., Baldacci, G., Burgers, P.M. and Hübscher, U. (2001) A unified nomenclature for the subunits of eukaryotic DNA polymerase delta. Trends Biochem. Sci. 26, 16-17. PubMed

Gray, F.C. and MacNeill, S.A. (2000) The Schizosaccharomyces pombe rfc3 gene encodes a homologue of the human hRFC36 and S.cerevisiae Rfc3 subunits of replication factor C. Curr. Genet. 37, 159-167. PubMed

Reynolds, N., Warbrick, E., Fantes, P.A. and MacNeill, S.A. (2000) Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21Cip1-like PCNA binding motif. EMBO J. 19, 1108-1118. PubMed

Kang, H.-Y., Choi, E., Bae, S.-H., Lee, K.-H., Gim, B.-S., Kim, H.-D., Park, C., MacNeill, S.A. and Seo, Y.-S. (2000) Genetic analyses of Schizosaccharomyces pombe dna2 reveal that Dna2 plays an essential role in Okazaki fragment metabolism. Genetics 155, 1055-1067. PubMed

MacNeill, S.A. and Burgers, P.M.J. (2000) Chromosomal DNA replication in yeast: enzymes and mechanisms. In The Yeast Nucleus: Frontiers in Molecular Biology, Oxford University Press, UK.

Reynolds, N., Fantes, P.A. and MacNeill, S.A. (1999) A key role for replication factor C in DNA replication checkpoint control in fission yeast. Nucl. Acids Res. 27, 462-469. PubMed

Reynolds, N. and MacNeill, S.A. (1999) Characterisation of XCdc1, a Xenopus homologue of the small subunit of DNA polymerase delta : identification of conserved regions (I – X) based on protein sequence comparisons across ten eukaryotic species. Gene 230, 15-22. PubMed

Reynolds, N., Watt, A., Fantes, P.A. and MacNeill, S.A. (1998) Cdm1, the smallest subunit of DNA polymerase delta in the fission yeast S.pombe, is non-essential for growth and division. Curr. Genet. 34, 250-258. PubMed

Zuo, S.-J., Gibbs, E., Kelman, Z., Wang, T.S.F., O’Donnell, M., MacNeill, S.A. and Hurwitz, J. (1997) DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits. Proc. Natl. Acad. Sci. USA 94, 11244-11249. PubMed

MacNeill, S.A. and Nurse, P. (1997) Cell cycle control in fission yeast. In The Molecular and Cellular Biology of Yeast, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, USA.

MacNeill, S.A. and Fantes, P. (1997) Genetic and physiological analysis of DNA replication in fission yeast. Meths. Enzymol. 283, 440-459. PubMed

MacNeill, S.A., Moreno, S., Reynolds, N., Nurse, P. and Fantes, P.A. (1996) The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27. EMBO J. 15, 4613-4628. PubMed

Reed, S., Hutchison, C.J.. and MacNeill, S.A. (1995) A brief history of the cell cycle. In Frontiers in Molecular Biology: The Cell Cycle, p.16-39, IRL Press, Oxford, U.K. Oxford University Press

MacNeill, S.A. (1994) Experiments with fission yeast. Genetics Research 63, 233-234. Cambridge University Press

MacNeill, S.A. (1994) Molecular mechanisms of cell cycle control in yeast. In The Mycota: Growth, Differentiation and Sexuality, p. 3-23, Spinger-Verlag, Heidelberg, Germany.

MacNeill, S.A. and Fantes, P.A. (1995) New roles for RNA in fission yeast. Curr. Biol. 5, 4-6. PubMed

MacNeill, S.A. and Fantes P.A. (1994) Methods for analysis of the fission yeast cell cycle. In The Cell Cycle: A Practical Approach, p. 93-125, IRL Press, Oxford, U.K.

MacNeill, S.A. and Nurse, P. (1993) Genetic analysis of human p34CDC2 function in fission yeast. Mol. Gen. Genet. 240, 315-322. PubMed

MacNeill, S.A. and Nurse P. (1993) Mutational analysis of the fission yeast p34cdc2 protein kinase gene. Mol. Gen. Genet. 236, 415-426. PubMed

Ayscough, K., Hayles, J., MacNeill, S.A. and Nurse, P. (1992) Cold-sensitive mutants of p34cdc2 that suppress a mitotic catastrophe phenotype in fission yeast. Mol. Gen. Genet. 232, 344-350. PubMed

Hughes, D., MacNeill, S.A. and Fantes, P.A. (1992) Molecular cloning and sequence analysis of cdc27 required for the G2-M transition in the fission yeast Schizosaccharomyces pombe. Mol. Gen. Genet. 231, 401-410. PubMed

MacNeill, S.A., Warbrick, E. and Fantes, P.A. (1991) Controlling cell cycle progress in the fission yeast Schizosaccharomyces pombe. Curr. Op. Genet. Devel. 1, 307-312. PubMed

Fantes, P.A., Warbrick, E., Hughes, D.A. and MacNeill, S.A. (1991) New elements in the mitotic control of fission yeast Schizosaccharomyces pombe. Cold Spring Harbor Symp. Quant. Biol. 56, 605-611. PubMed

MacNeill, S.A., Creanor, J. and Nurse, P. (1991) Isolation, characterisation and molecular cloning of new mutant alleles of the fission yeast p34cdc2 protein kinase: identification of new temperature-sensitive G2-arresting alleles. Mol. Gen. Genet. 229, 109-118. PubMed

Iggo, R.D., Jamieson, D.J., MacNeill, S.A., Southgate, J., McPheat, J. and Lane, D. (1991) p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts. Mol. Cell. Biol. 11, 1326-1333. PubMed

Paris, J., LeGuellic, R., Couturier, A., LeGuellic, K., Omilli, F., Camonis, J., MacNeill, S.A. and Philippe, M. (1991) Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2. Proc. Natl. Acad. Sci. USA 88, 1039-1043. PubMed

MacNeill, S.A. and Nurse, P. (1989) Genetic interactions in the control of mitosis in fission yeast. Curr. Genet., 16, 1-16. PubMed

Carr, A.M., MacNeill, S.A., Hayles, J. and Nurse, P. (1989) Sequence analysis of mutant alleles of the fission yeast cdc2 protein kinase gene: implications for cdc2 protein structure and function. Mol. Gen. Genet. 218, 41-49. PubMed

Simanis, V., Carr, A.M., Goss, M., Lee, M.G., MacNeill, S.A. and Nurse, P. (1987) Cell cycle regulation in yeasts and man: towards a unifying mechanism. Antonie Van Leeuwenhoek, 53, 319-23. PubMed